African 2, a clonal complex of Mycobacterium bovis epidemiologically important in East Africa.

نویسندگان

  • Stefan Berg
  • M Carmen Garcia-Pelayo
  • Borna Müller
  • Elena Hailu
  • Benon Asiimwe
  • Kristin Kremer
  • James Dale
  • M Beatrice Boniotti
  • Sabrina Rodriguez
  • Markus Hilty
  • Leen Rigouts
  • Rebuma Firdessa
  • Adelina Machado
  • Custodia Mucavele
  • Bongo Nare Richard Ngandolo
  • Judith Bruchfeld
  • Laura Boschiroli
  • Annélle Müller
  • Naima Sahraoui
  • Maria Pacciarini
  • Simeon Cadmus
  • Moses Joloba
  • Dick van Soolingen
  • Anita L Michel
  • Berit Djønne
  • Alicia Aranaz
  • Jakob Zinsstag
  • Paul van Helden
  • Françoise Portaels
  • Rudovick Kazwala
  • Gunilla Källenius
  • R Glyn Hewinson
  • Abraham Aseffa
  • Stephen V Gordon
  • Noel H Smith
چکیده

We have identified a clonal complex of Mycobacterium bovis isolated at high frequency from cattle in Uganda, Burundi, Tanzania, and Ethiopia. We have named this related group of M. bovis strains the African 2 (Af2) clonal complex of M. bovis. Af2 strains are defined by a specific chromosomal deletion (RDAf2) and can be identified by the absence of spacers 3 to 7 in their spoligotype patterns. Deletion analysis of M. bovis isolates from Algeria, Mali, Chad, Nigeria, Cameroon, South Africa, and Mozambique did not identify any strains of the Af2 clonal complex, suggesting that this clonal complex of M. bovis is localized in East Africa. The specific spoligotype pattern of the Af2 clonal complex was rarely identified among isolates from outside Africa, and the few isolates that were found and tested were intact at the RDAf2 locus. We conclude that the Af2 clonal complex is localized to cattle in East Africa. We found that strains of the Af2 clonal complex of M. bovis have, in general, four or more copies of the insertion sequence IS6110, in contrast to the majority of M. bovis strains isolated from cattle, which are thought to carry only one or a few copies.

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عنوان ژورنال:
  • Journal of bacteriology

دوره 193 3  شماره 

صفحات  -

تاریخ انتشار 2011